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Alomone Labs
anti human zip8 ![]() Anti Human Zip8, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more https://www.bioz.com/result/anti human zip8/product/Alomone Labs Average 91 stars, based on 1 article reviews
anti human zip8 - by Bioz Stars,
2026-03
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Merck & Co
zip8 ![]() Zip8, supplied by Merck & Co, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more https://www.bioz.com/result/zip8/product/Merck & Co Average 90 stars, based on 1 article reviews
zip8 - by Bioz Stars,
2026-03
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GenScript corporation
monoclonal mouse anti-zip8 antibody (9d4a9) ![]() Monoclonal Mouse Anti Zip8 Antibody (9d4a9), supplied by GenScript corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more https://www.bioz.com/result/monoclonal mouse anti-zip8 antibody (9d4a9)/product/GenScript corporation Average 90 stars, based on 1 article reviews
monoclonal mouse anti-zip8 antibody (9d4a9) - by Bioz Stars,
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Image Search Results
Journal: bioRxiv
Article Title: Discovery of a Selective Inhibitor of ZIP14 with Therapeutic Potential for Cancer-associated Cachexia
doi: 10.1101/2025.10.23.682519
Figure Lengend Snippet: Xenopus oocytes injected either with ( A, B ) human ZIP14 or ( E, F ) human ZIP8 cRNA were incubated with either ( A, E ) ⁶⁵Zn or ( B, F ) ⁵⁵Fe in the presence of ( A, E ) 50 µM or ( B, F ) 10 µM PPTD for 30 min at 22 °C. Radioactivity was measured as described under Methods . Data are normalized against DMSO-treated transporter-expressing controls, and presented as mean ± standard deviation (S.D.) (n=3 independent experiments). Similarly, (C, D) TREx-hZIP14 or (G, H) TREx-hZIP8 cells were treated with Tet (1 μg/mL) for 24 h, followed by incubation either with ( C, G ) ⁵⁴MnCl₂ or (D, H) ¹⁰⁹CdCl₂ in the presence of PPTD at the indicated concentrations for 1 h. Radioactivity was measured as described under Methods . The data (mean + SD) represent results from three independent experiments. Statistical significance was determined using Student’s t -test (A, B, E, F) or one-way ANOVA followed by Dunnett’s post hoc test (C, D, G, H). (* p < 0.05, ** p < 0.01, *** p < 0.005, **** p < 0.001, ***** p < 0.0005).
Article Snippet: The following primary antibodies were used: anti-human ZIP14 (generated previously ),
Techniques: Injection, Incubation, Radioactivity, Expressing, Standard Deviation
Journal: Blood Advances
Article Title: A mouse model characterizes the roles of ZIP8 in systemic iron recycling and lung inflammation and infection
doi: 10.1182/bloodadvances.2022007867
Figure Lengend Snippet: ZIP8 KO mice have decreased liver manganese and increased splenic iron levels. ICP-MS was performed on liver and spleens from 16-week-old ZIP8 KO mice and littermate controls (fl/fl). n = 5 mice per group. (A,C) Concentrations and (B,D) total levels of (A-B) liver and (C-D) splenic iron, manganese, and zinc were measured. Graphs depict mean and SD; P values determined by 2-tailed unpaired Student t test. SD, standard deviation.
Article Snippet:
Techniques: Standard Deviation
Journal: Blood Advances
Article Title: A mouse model characterizes the roles of ZIP8 in systemic iron recycling and lung inflammation and infection
doi: 10.1182/bloodadvances.2022007867
Figure Lengend Snippet: Baseline ZIP8 KO mice have impaired iron recycling. (A-D) Tissues and serum from 16-week-old ZIP8 KO and littermate controls (fl/fl) were harvested for analysis. n = 4 to 14 mice per group. (A) Liver, spleen and serum nonheme iron levels. (B) CBCs. (C) Liver Hamp mRNA and BM Erfe mRNA levels. (D) Representative images of Perls’ staining for iron in ZIP8 KO and fl/fl FFPE spleen tissue. 40× original magnification. (E) Splenic red pulp macrophages from C57BL/6 mice were isolated via magnetic bead selection for F4/80+ cells. ZIP8 protein levels are enriched in the splenic F4/80+ population as determined by western blot. n = 4 mice per group. (A-C) Graphs depict mean and SD; P values determined by 2-tailed unpaired Student t test. BM, bone marrow; FFPE, formalin-fixed paraffin-embedded; SD, standard deviation.
Article Snippet:
Techniques: Staining, Isolation, Selection, Western Blot, Formalin-fixed Paraffin-Embedded, Standard Deviation
Journal: Blood Advances
Article Title: A mouse model characterizes the roles of ZIP8 in systemic iron recycling and lung inflammation and infection
doi: 10.1182/bloodadvances.2022007867
Figure Lengend Snippet: ZIP8 KO and WT mice have similar responses to PHZ. (A-C) C57BL/6 mice were treated with 300 μL of aged RBCs, a single IP injection of 10 mg iron dextran or 2 consecutive daily doses of 60 mg/kg PHZ to induce hemolytic anemia. n = 3 to 5 mice for each treatment group. Tissues were harvested 3 days after treatment. n = 3 to 5 mice per group. (A) Western blot of splenic ZIP8. (B) Correlation between splenic ZIP8 protein levels as quantified by densitometry and splenic nonheme iron levels. P value and R 2 as determined by Pearson’s correlation. (C) Liver Saa1 mRNA levels. (D-F) ZIP8 KO and littermate controls (fl/fl) were treated with PHZ as described above. n = 6 to 9 mice per group. (D) CBCs were performed on day 4 and day 7 on heparinized blood from ZIP8 KO and fl/fl mice. (E) Day 7 liver Saa1 , liver Nqo1 , and spleen Nqo1 mRNA levels and (F) spleen and serum nonheme iron. (A,C-F) Graphs depict mean and SD; P values determined by 2-tailed unpaired Student t test. SD, standard deviation; WT, wild-type.
Article Snippet:
Techniques: Injection, Western Blot, Standard Deviation
Journal: Blood Advances
Article Title: A mouse model characterizes the roles of ZIP8 in systemic iron recycling and lung inflammation and infection
doi: 10.1182/bloodadvances.2022007867
Figure Lengend Snippet: Loss of ZIP8 has no effect on response to iron deficiency in mice. ZIP8 KO mice and littermate controls (fl/fl) were placed on a 4 ppm Fe diet and CBCs were measured at 6, 8, 12, 16, 20, and 24 weeks on the diet. Tissues and serum were harvested at the end of the treatment. n = 8 to 9 mice per group. (A) CBCs from ZIP8 KO and fl/fl mice. (B) Blood ZPP levels. (C) Spleen, liver, and serum nonheme iron levels from iron-deficient ZIP8 KO mice and fl/fl mice. (D) Liver Hamp1 mRNA. (E) Representative western blot for splenic Fpn, Tfr1, and FtnH protein. (A-D) Graphs depict mean and SD. SD, standard deviation; ZPP, zinc protoporphyrin.
Article Snippet:
Techniques: Western Blot, Standard Deviation
Journal: Blood Advances
Article Title: A mouse model characterizes the roles of ZIP8 in systemic iron recycling and lung inflammation and infection
doi: 10.1182/bloodadvances.2022007867
Figure Lengend Snippet: ZIP8 is induced by inflammation in human alveolar epithelial cells. (A) Representative images for IHC for ZIP8 (brown) and SP-C (red; AT2 cells) in normal human lung tissue (n = 2 biological replicates). Images are 10× (left) and 40× (right) original magnification. (B-D) A549 cells (human alveolar epithelial cell line) were serum starved for 24 hours, then treated with 50 ng/mL recombinant human IL-6, IFN-γ, IL-1α, IL-1β, TNFα, or epidermal growth factor for 16 hours. n = 1 to 3 independent experiments. (B) Representative western blot for ZIP8 protein expression. (C) SLC39A8 mRNA levels. (D) ZIP8 siRNA knockdown. (E) 16HBE cells (human bronchiolar epithelial cell line) SLC39A8 mRNA levels after treatment for 16 hours. Graphs depict mean and SD. IFN- γ, interferon gamma; KD, ZIP8 siRNA; NT, nontargeting siRNA; SD, standard deviation.
Article Snippet:
Techniques: Recombinant, Western Blot, Expressing, Knockdown, Standard Deviation
Journal: Blood Advances
Article Title: A mouse model characterizes the roles of ZIP8 in systemic iron recycling and lung inflammation and infection
doi: 10.1182/bloodadvances.2022007867
Figure Lengend Snippet: ZIP8 transports iron from the airspace into the lung. (A) Slc39a8 mRNA levels of C57BL/6 mouse lung subpopulation cell types. B CD45+: BAL macrophages. L CD45+: lung macrophages. CD31+: endothelial cells. Epcam+: epithelial cells. n = 3 to 4 mice per group. (B) ZIP8 KO mice and littermate controls (fl/fl) were treated with 5 μg of 58 Fe 2+ via RO injection or OP aspiration and harvested after 4 hours for ICP-MS analysis of 58 Fe levels. n = 3 mice per group. (C-D) 16-week-old ZIP8 KO and fl/fl mouse lung (C) nonheme iron levels and (D) Nqo1 mRNA and MDA levels. n = 4 to 10 mice per group. (A-D) Graphs depict mean and SD; P values were determined by 2-tailed unpaired Student t test. MDA, malondialdehyde; SD, standard deviation.
Article Snippet:
Techniques: Injection, Standard Deviation
Journal: Blood Advances
Article Title: A mouse model characterizes the roles of ZIP8 in systemic iron recycling and lung inflammation and infection
doi: 10.1182/bloodadvances.2022007867
Figure Lengend Snippet: ZIP8 does not play a significant role in LPS-induced ALI. 16-week-old ZIP8 KO mice and littermate controls (fl/fl) were treated with 15 mg/kg LPS through OP aspiration to induce ALI and harvested after 3 days. A subset of mice in both groups were left untreated for comparison. n = 3 to 7 mice per group. (A) Representative western blot for ZIP8 in lung lysate. (B) Lung nonheme iron levels. (C) BAL protein levels. (D) BAL and lung tissue MPO activity. (E) Lung mitochondrial SOD. (B-E) Graphs depict mean and SD. SD, standard deviation.
Article Snippet:
Techniques: Comparison, Western Blot, Activity Assay, Standard Deviation
Journal: Blood Advances
Article Title: A mouse model characterizes the roles of ZIP8 in systemic iron recycling and lung inflammation and infection
doi: 10.1182/bloodadvances.2022007867
Figure Lengend Snippet: ZIP8 deletion is protective against K pneumoniae infection. ZIP8 KO mice and littermate controls (fl/fl) were infected with 1500 CFU of K pneumoniae via OP aspiration and weighed daily until harvest at 3 days. (A) Percent weight loss in ZIP8 KO and fl/fl mice. n = 3 to 4 mice per group. (B) CFU counts in lung, liver, spleen, and blood after infection. (C) BAL protein levels. (D) BAL and lung tissue MPO activity. (E) Lung mitochondrial and cytosolic SOD. Graphs depict mean and SD; P values were determined by 2-tailed unpaired Student t test. SD, standard deviation.
Article Snippet:
Techniques: Infection, Activity Assay, Standard Deviation